LOCUS       40924_31720       13937 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pMpGWB318 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13937)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T.
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha
  JOURNAL   PLoS ONE 10 (9), E0138876 (2015)
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13937)
  AUTHORS   Nishihama R, Ishizaki K, Kohchi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, 
            Graduate School of Biostudies; Kitashirakawa-oiwake-cho, Sakyo-ku, 
            Kyoto, Kyoto 606-8502, Japan URL :http://www.lif.kyoto-u.ac.jp/e/
REFERENCE   3  (bases 1 to 13937)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13937)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "9"; pages: "E0138876"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, Graduate 
            School of Biostudies"; volume: " Kitashirakawa-oiwake-cho, Sakyo-ku,
            Kyoto, Kyoto 606-8502, Japan URL :http"; pages: 
            "//www.lif.kyoto-u.ac.jp/e"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      315..2043
                     /label=MpEF1alpha promoter and 5'UTR
                     /note="MpEF1alpha promoter and 5'UTR"
                     /regulatory_class="promoter"
     protein_bind    2057..2181
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2218..2248
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2302..2958
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             3303..3605
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(3649..3773)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    3784..3819
                     /note="modified EAR motif plant-specific repression domain 
                     showing strong repression activity (SRDX)"
     terminator      3844..4096
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4130..4146)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4154..4170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4178..4208)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4223..4244)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     polyA_signal    complement(4329..4504)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4512..6548)
                     /codon_start=1
                     /gene="mALS"
                     /product="acetolactate synthase"
                     /label=mALS
                     /note="from Marchantia polymorpha; contains triple
                     amino-acid subustitutions (P207S/R208S/W582L)"
                     /protein_id="BAS49938.1"
                     /translation="MASISGCAAVPLSIASLEKVQPSARSCCKGAKELKVRSGFFSGAA
                     LRGHAYNTSRKTERSLEVKAQAVSEKIQSAVDVSGPRLSSGKSLVAPTPSDFQSRYKDD
                     EPRKGADILVEALEREGIRTVFAYPGGASMEIHQALTRSSSIRNILCRHEQGEIFAAEG
                     YAKSSGRVGVCIATSGPGATNLVTGLADALLDSVPLVAITGQVSSRMIGTDAFQETPIV
                     EVTRSITKHNYLVMNIDDIPRVIREAFFLAASGRPGPVLVDIPKDVQQQMAIPDWSQPM
                     QLKGYLQRLPRPPEPTLIEQIIRLISSSKKPVVYSGGGCNHASEELREFVELTGIPITS
                     TLMGLGAYPESDPLSLKMLGMHGTVYANYAVDQADLLLAFGVRFDDRVTGKLETFASRS
                     SIVHIDIDPAEIGKNKQPHVSMCADVKPALSEINALLKSQKRTFDFSNWVAELEEQKQK
                     WPMKFPVFEDAIAPQSAIQTLYELTGGNAIISTGVGQHQMWAAQWFKYNEPRRWLTSGG
                     LGAMGFGLPSALGAAATQPDIPVIDIDGDGSFVMNVQELATIFVEKLPVKIMILNNQHL
                     GMVVQLEDRFYKANRAHTYLGDPDHEEQIYPDFCTVADGFKVPNARVVKKDELRAAIQT
                     MLDSPGPYLLDVIVPHQEHVLPMIPGGASFKEIITEGDGRTIY"
     gene            complement(4512..6548)
                     /gene="mALS"
                     /label=mALS
     promoter        complement(6588..7264)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    complement(7459..7483)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8004..8792
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      9041..9629
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(9815..9955)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10299..10493)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10562..11632)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(12064..12690)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag aagaaagcaa
      901 cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta gtccaccgtt
      961 tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg gccacctctc
     1021 tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac tcgaaaaacc
     1081 ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc ttattttgga
     1141 atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga cggaggagtg
     1201 tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga aggggcatgg
     1261 acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc gtcgtcggcc
     1321 tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg gccgccgacc
     1381 ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc tcgttattat
     1441 aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc gctaatcatt
     1501 ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc aaagctcgct
     1561 gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt tcctttcact
     1621 tctttctctc ttgcgtggag agcatcgaca tcgaagagct gcatcttgtc ttctggtatg
     1681 cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga ttgtctagac
     1741 ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga gtgatttttt
     1801 aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg gtaaggtgtt
     1861 cagttcggtc tctggtgcgg aggtgcctcg taagtttcct gcatgataga tttgtctatc
     1921 cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg acgtcggtgc
     1981 tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc tgcagaaagg
     2041 ttgactagag ttatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
     2101 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     2161 acaacatatc cagtcactat ggcggcccct cgaggggccg cattaggcac cccaggcttt
     2221 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc ggcgagattt
     2281 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
     2341 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
     2401 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
     2461 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
     2521 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     2581 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
     2641 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     2701 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
     2761 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     2821 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     2881 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
     2941 gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag ataacagtat
     3001 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
     3061 atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct
     3121 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
     3181 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
     3241 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg
     3301 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     3361 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
     3421 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     3481 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     3541 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     3601 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
     3661 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     3721 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgataac
     3781 agcttggatt tggatttgga gttgcgcttg ggattcgcct aggcttagag ctcgaatttc
     3841 cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     3901 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     3961 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     4021 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     4081 tctatgttac tagatcggga attggttccg gaaccaattc gtaatcatgg tcatagctgt
     4141 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4201 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaggctga attaggcgcg
     4261 cctatttctg aattcaacac attgcggacg tttttaatgt actgaattaa cgccgaatta
     4321 attcggggga tctggatttt agtactggat tttggtttta ggaattagaa attttattga
     4381 tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc aacacatgag
     4441 cgaaacccta taggaaccct aaattccctt atctgggaac tactcacaca ttattatgga
     4501 gaaactcgaa gttagtatat agtccttcca tcaccctctg tgataatctc cttaaaactt
     4561 gctcccccag gaatcatagg cagaacgtgc tcctggtggg gaactatcac atccagtaaa
     4621 taaggaccgg gagaatctag catagtttga attgctgctc ttagttcatc cttcttcacc
     4681 acacgcgcat ttggcacctt gaaaccgtct gcaactgtac agaagtcggg gtagatctgc
     4741 tcctcgtgat ctggatcacc taagtaagta tgtgctctgt tggccttgta gaatctatcc
     4801 tccaattgta cgaccattcc tagatgttgg ttgtttaaga tcatgatctt cacgggtaac
     4861 ttctccacaa aaattgtagc caactcctgt acgttcatca caaagctacc atcgccatcg
     4921 atgtcgatca caggaatatc tggttgagtt gcggcagccc ccaaagcaga aggaagtccg
     4981 aatcccatgg ctccaagacc tccagatgtc aaccaacgcc gcggttcgtt gtacttgaac
     5041 cactgggctg cccacatttg atgttggcca acaccagtag atatgatggc atttccacca
     5101 gtgagttcgt acagcgtctg tatggcagat tgaggagcaa tggcatcctc gaatacggga
     5161 aacttcattg gccatttctg cttttgttct tctagttctg ccacccagtt ggagaagtcg
     5221 aaagtcctct tttgactctt caagagggca ttgatttcac ttagcgcagg tttcacatca
     5281 gcacacatgg acacatgggg ctgtttgttc tttccaattt cagcagggtc aatatcgata
     5341 tgaacgatgc tggacctgct agcaaaagtc tccagtttac cagtcactcg atcgtcgaaa
     5401 cgcactccaa atgccaacaa cagatctgct tggtccacgg cgtagttggc gtaaacagtt
     5461 ccatgcattc caagcatttt caaacttaat ggatcagact cgggatacgc tcccaaaccc
     5521 attaaagtcg atgtgatagg aattccggta agctcgacaa attccctcaa ctcttcgctg
     5581 gcgtgattgc aacctcctcc agaatacact acaggcttct tagaactaga aatgagtctt
     5641 ataatctgct cgattaacgt tggttccggt ggccgaggca gtcgttgtaa gtagcccttc
     5701 agttgcattg gctgagacca atctggaata gccatttgct gttgcacgtc cttgggaata
     5761 tctaccaaga ctgggcccgg cctacctgaa gctgccaaaa agaaagcctc cctgattacg
     5821 cgagggatgt catcaatgtt cataacaagg taattgtgct tggtgatcga tctcgtaacc
     5881 tccacaattg gagtttcttg aaaagcatcg gtaccaatca tcctactgga tacttgacct
     5941 gtaatggcca ctagtggcac actgtccaag agggcatcag cgagcccggt aacaagattg
     6001 gtcgctccag gaccggatgt ggctatacaa acccctactc gtccagacga tttagcataa
     6061 ccttcagcag cgaaaatctc cccctgctcg tggcgacaga gaatgttgcg gatgctgctg
     6121 gaacgtgtaa gagcctggtg aatctccata gacgctccgc cgggataagc gaacaccgtt
     6181 ctaatccctt ctcgctcaag agcctccacc aaaatgtcgg ctcctttgcg gggctcgtca
     6241 tccttgtagc gagactgaaa gtcggacggg gttggtgcaa cgagtgactt cccgctgcta
     6301 agccgtggtc cactcacatc gactgcagac tggatcttct cgctaacagc ttgggctttc
     6361 acttccagtg aacgctcagt cttccgagag gtgttgtatg catgtcccct cagagcggct
     6421 cccgagaaga aaccactcct cacctttagc tctttagctc ccttgcaaca agaccttgcc
     6481 gatggctgca ccttctccag gctcgctatg ctcaaaggca cagctgcgca tccactaatt
     6541 gacgccatgc tcgagagaga tagatttgta gagagagact ggtgatttca gcgtgtcctc
     6601 tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata gtgggattgt
     6661 gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt
     6721 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
     6781 tcggcagagg catcttgaac gatagccttt cctttatcgc aattatggca tttgtaggtg
     6841 ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg
     6901 aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag
     6961 actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat gttcacatca
     7021 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
     7081 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
     7141 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
     7201 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
     7261 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
     7321 gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgaa ttcagaaata
     7381 aattcagcct aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag
     7441 ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct
     7501 ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga
     7561 cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt
     7621 cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca
     7681 tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt
     7741 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     7801 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     7861 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     7921 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     7981 gccctaaaac aaagttaaac atcatggggg aagcggtgat cgccgaagta tcgactcaac
     8041 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     8101 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     8161 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     8221 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     8281 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     8341 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     8401 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     8461 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     8521 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     8581 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     8641 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     8701 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     8761 tgaaaggcga gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc
     8821 cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct
     8881 cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc
     8941 tacacaaatt gggagatata tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc
     9001 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     9061 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     9121 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     9181 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     9241 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     9301 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     9361 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9421 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9481 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9541 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9601 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9661 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9721 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     9781 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     9841 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     9901 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
     9961 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
    10021 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
    10081 gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg
    10141 cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg
    10201 ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg
    10261 ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt
    10321 taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta
    10381 tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg
    10441 ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga
    10501 gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt
    10561 aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc
    10621 cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct
    10681 tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg
    10741 tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa
    10801 aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt
    10861 tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg
    10921 gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata
    10981 ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt
    11041 ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc
    11101 agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc
    11161 cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt
    11221 aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa
    11281 gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca
    11341 cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat
    11401 ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt
    11461 gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt
    11521 ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat
    11581 cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc
    11641 ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc cgcttacgcc
    11701 tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc
    11761 ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc
    11821 tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt
    11881 ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc
    11941 gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc
    12001 tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac
    12061 tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc
    12121 ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg
    12181 ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc
    12241 accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc
    12301 atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc
    12361 gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc
    12421 caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt
    12481 cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg
    12541 cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc
    12601 cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc
    12661 tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact
    12721 catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac
    12781 ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc
    12841 ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc
    12901 ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac
    12961 ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg
    13021 cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg
    13081 cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca
    13141 cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg
    13201 cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct
    13261 tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa
    13321 tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct
    13381 ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt
    13441 cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta
    13501 ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg
    13561 aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt
    13621 cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt
    13681 aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa
    13741 aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt
    13801 cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg
    13861 gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg
    13921 gacgaacgga taaacct
//
